THIOLYTE(R) MB
mBBr [Monobromobimane]
CAS: 71418-44-5
Molecular Formula: C10H11BrN2O2
THIOLYTE(R) MB - Names and Identifiers
THIOLYTE(R) MB - Physico-chemical Properties
| Molecular Formula | C10H11BrN2O2 |
| Molar Mass | 271.11 |
| Density | 1.66±0.1 g/cm3(Predicted) |
| Melting Point | 152-154°C(lit.) |
| Boling Point | 327.8±44.0 °C(Predicted) |
| Solubility | DMF: soluble |
| Appearance | Morphological powder |
| Color | Yellow |
| Maximum wavelength(λmax) | 398 nm |
| BRN | 4430959 |
| pKa | -3.36±0.70(Predicted) |
| Storage Condition | 2-8°C |
| Stability | Light Sensitive |
| MDL | MFCD00036951 |
| In vitro study | Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols [e.g., the tripeptide thiol glutathione (GSH)], and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organic solvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and the anionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes are yellow. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols. |
THIOLYTE(R) MB - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 8 |
THIOLYTE(R) MB - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.689 ml | 18.443 ml | 36.885 ml |
| 5 mM | 0.738 ml | 3.689 ml | 7.377 ml |
| 10 mM | 0.369 ml | 1.844 ml | 3.689 ml |
| 5 mM | 0.074 ml | 0.369 ml | 0.738 ml |
Last Update：2024-01-02 23:10:35
THIOLYTE(R) MB - Nature
| maximum wavelength (& lambda;max) | 398 nm |
| biological applications | Glutathione S-transferase substrates; detecting glutathione S-transferase,thiols,sulfite,homocysteine,mycothiol,sulfur compounds; thiol-reactive probes |
Last Update：2024-04-10 22:29:15
THIOLYTE(R) MB - Uses and synthesis methods
biological activity
Bromobimane is basically a non-fluorescent substance, and when reacted with small mercaptan, fluorescent products are generated.
in vitro studies
Bromobimanes in solution (aqueous or organic solvents of medium polarity) react with small thiols [e.g., the tripeptide thiol glutathione (GSH)], and with reactive protein thiol groups (e.g., hemoglobin). The reactions of bromobimanes with thiols are second order and dependent on pH, the active nucleophile being the thiolate anion. The reaction of bromobimane with a thiolate converts the nonfluorescent agent into water-soluble fluorescent products. The neutral agents mBBr and bBBr are moderately soluble in mediumpolarity organic solvents (acetonitrile, dichloromethane), and slightly soluble in water. The quaternary salt, qBBr, and the anionic bromobimane, SBBr, are soluble in water, but less soluble in organic solvents. Bromobimanes are yellow. The highly selective, rapid reactivity of bromobimanes toward thiols, the stability and fluorescence yield of the thiol derivatives, the ease of separation of the derivatives by reversed-phase HPLC, and the availability of both cell-penetrating and nonpenetrating forms, make the use bromobimanes an extremely powerful approach to the analysis of low molecular weight biothiols.
Last Update：2024-04-10 22:29:15
THIOLYTE(R) MB - Introduction
mBBr [Monobromobimane] is a class of chemical reagents used to detect and measure fluorescent substances. They have the following properties:
1. Fluorescence: mBBr [Monobromobimane] can absorb light of a specific wavelength and emit fluorescent light of different wavelengths, so that the measured substance has a specific peak in the fluorescence spectrum.
2. Sensitivity: These reagents have high sensitivity to trace amounts of fluorescent substances and can detect low concentrations of targets.
3. Specificity: mBBr [Monobromobimane] can be combined with specific fluorescent substances, and has less interference with non-target substances, thereby improving the accuracy of the measurement.
mBBr [Monobromobimane] is mainly used in the following areas:
1. Biological Sciences: Fluorescent dyes and indicators are widely used in cell and tissue imaging, protein fluorescence labeling and so on.
2. Environmental Science: mBBr [Monobromobimane] is used for the determination of fluorescent substances in environmental samples such as water and atmosphere, and can monitor the presence and concentration of environmental pollutants.
3. medical diagnosis: mBBr [Monobromobimane] is used for the preparation of biomarkers and fluorescent probes, which can assist in the diagnosis and detection of diseases.
The preparation method of mBBr [Monobromobimane] can be determined according to the specific reagent type and application purpose. The common preparation methods include chemical synthesis and high purity extraction.
When using mBBr [Monobromobimane], you need to pay attention to the following safety information:
1. Follow laboratory safety practices and wear personal protective equipment (e. g., lab gloves, goggles, etc.).
2. Avoid skin contact and inhalation of reagents. If there is accidental contact, rinse with water immediately.
3. operate in strict accordance with the instructions for use and safety data sheets of reagents to avoid misuse and mixing of reagents.
4. when storing the reagent, it is necessary to avoid light and high temperature environment, and properly mark the composition and storage date of the reagent.
1. Fluorescence: mBBr [Monobromobimane] can absorb light of a specific wavelength and emit fluorescent light of different wavelengths, so that the measured substance has a specific peak in the fluorescence spectrum.
2. Sensitivity: These reagents have high sensitivity to trace amounts of fluorescent substances and can detect low concentrations of targets.
3. Specificity: mBBr [Monobromobimane] can be combined with specific fluorescent substances, and has less interference with non-target substances, thereby improving the accuracy of the measurement.
mBBr [Monobromobimane] is mainly used in the following areas:
1. Biological Sciences: Fluorescent dyes and indicators are widely used in cell and tissue imaging, protein fluorescence labeling and so on.
2. Environmental Science: mBBr [Monobromobimane] is used for the determination of fluorescent substances in environmental samples such as water and atmosphere, and can monitor the presence and concentration of environmental pollutants.
3. medical diagnosis: mBBr [Monobromobimane] is used for the preparation of biomarkers and fluorescent probes, which can assist in the diagnosis and detection of diseases.
The preparation method of mBBr [Monobromobimane] can be determined according to the specific reagent type and application purpose. The common preparation methods include chemical synthesis and high purity extraction.
When using mBBr [Monobromobimane], you need to pay attention to the following safety information:
1. Follow laboratory safety practices and wear personal protective equipment (e. g., lab gloves, goggles, etc.).
2. Avoid skin contact and inhalation of reagents. If there is accidental contact, rinse with water immediately.
3. operate in strict accordance with the instructions for use and safety data sheets of reagents to avoid misuse and mixing of reagents.
4. when storing the reagent, it is necessary to avoid light and high temperature environment, and properly mark the composition and storage date of the reagent.
Last Update：2024-04-09 21:21:28
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Spot supply
Product Name: mBBr [Monobromobimane] Visit Supplier Webpage Request for quotationCAS: 71418-44-5
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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